Recombinant:Article Title: Nuclear receptor E75/NR1D2 drives tumor malignant transformation by integrating Hippo and Notch pathways
Article Snippet: Primary antibodies used include mouse Anti-beta Galactosidase (1:100, DSHB), mouse anti-Br (1:100, DSHB), rat anti- Elav (1:100, DSHB), mouse anti-Mmp-1 (1:100, DSHB), mouse anti-wg (1:100, DSHB), Cyclin E (1:100, Santa Cruz), rabbit anti-Dcp1 (CST, 1:100), mouse anti-dMyc (1:50, DSHB), mouse anti-Cut (1:100, DSHB), mouse anti-NICD (1:100, DSHB).
Article Title: Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila.
Article Snippet: This article is protected by copyright.. All rights reserved.. A cc ep te d A rt ic le Ashim Mukherjee ORCID iD: 0000-0002-3523-7089 Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila Vartika Sharma, Mousumi Mutsuddi, Ashim Mukherjee* Department of Molecular and Human Genetics, Institute of Science, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India *Corresponding Author Email: amukherjee@bhu.ac.in
Article Title: Microvilli-derived extracellular vesicles carry Hedgehog morphogenic signals for Drosophila wing imaginal disc development.
Article Snippet: Antibodies were used as follows: mouse anti-En (1:1000, Development Studies Hybridoma Bank University of Iowa – DSHB), rabbit anti-En (1:1000, Santa Cruz), mouse anti-Ptc42 (1/400), chicken anti-bgal (1:1000, Gen Tex), affinity-purified rabbit ‘‘Calvados’’ polyclonal anti-Hh43 (1:200), mouse anti-Cut (1:100, DSHB), mouse anti-Wg (1:50, DSHB), guinea pig anti-PromL (1:150, this study), mouse anti Dll44 (1:100), rabbit anti-aPKC (1:500, Santa Cruz), rat anti-DE-cadherin (1:50, DSHB), mouse anti-Dlg (1:100, DSHB);mouse anti-Arm (1:100,DSHB),mouse anti-GFP (1:1000, Roche), rabbit anti-Cad99c25 (1:1000, D. Godt, University of Toronto), rabbit anti-Caspase3 (1:500,CellSignaling).
Article Title: Body-fat sensor triggers ribosome maturation in the steroidogenic gland to initiate sexual maturation in Drosophila.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-Dlg DSHB Cat#4F3; RRID: AB_528203 mouse anti-Fibrillarin ThermoFisher Scientific Cat#38F3; RRID: AB_2532241 mouse anti-Lam Dm0 DSHB Cat#ADL84.12; RRID:AB_528338 mouse anti-Wg DSHB Cat#4D4; RRID: AB_528512 rabbit anti-Apolpp Joaquin Culi, Kutty et al., 1996 N/A rabbit anti-GFP Abcam Cat#Ab290 rabbit anti-IgG HRP-conjugated Sigma-Aldrich Cat# A9169 rabbit anti-ILP2 Hugo Stocker, Bader et al., 2013 N/A rabbit anti-Sema1a Alex Kolodkin, Yu et al., 1998 N/A rat anti-DE-Cad DSHB Cat#DCAD2; RRID: AB_528120 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31570; RRID: AB_2536180 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31572; RRID: AB_162543 donkey Anti-Rat IgG (H+L) Alexa Fluor 647 AffiniPure Jackson ImmunoResearch Cat#712-605-153; RRID: AB_2340694 Chemicals, peptides, and recombinant proteins Vectashield mounting medium with DAPI Vector Labs Cat#H-1200 DAPI-Fluoromount-G Electron Microscopy Sciences Cat#17984-24 GIBCO Schneider’s Drosophila Sterile Medium Thermo Fisher Cat#21720024 Bromophenol blue Sigma-Aldrich Cat#B6131 a-ecdysone Sigma-Aldrich Cat#9004 20-hydroxyecdysone Sigma-Aldrich Cat#H5142 SuperScript III First-Strand Synthesis System for RT-PCR Invitrogen Cat#18080-051 Power SYBR Green PCR Master Mix Applied Biosystems Cat#4367659 Paraformaldehyde 16% solution Electron Microscopy Sciences Cat#15710 Nile Red Sigma-Aldrich Cat#72485 1X cOmplete Mini EDTAfree protease inhibitor cocktail Sigma-Aldrich Cat#11836170001 Phalloidin 633 ThermoFisher Cat#A22284 Critical commercial assays 20-Hydroxyecdysone enzyme immunoassay kit Bertin Pharma Cat#A05120 Amplex Red Cholesterol Assay Kit ThermoFisher Scientific Cat#A12216 BCA Protein Assay Kit Pierce Cat#23227 Glucose (HK) Assay Kit Sigma-Aldrich Cat#GAHK20-1KT RNeasy-Mini Kit QIAGEN Cat#74106 RNase Free DNase Set QIAGEN Cat#79254 Experimental models: Organisms/strains D. melanogaster: mir-8NP5247-Gal4 DGRC 104917 D. melanogaster: NP2222-Gal4 DRGC 112830 D. melanogaster: phm22-Gal4 Kim Rewitz, Ono et al., 2006 N/A (Continued on next page) Cell Reports 37, 109830, October 12, 2021 e1
Article Title:
Article Snippet: Mouse anti-Antennapedia Developmental State Hybridoma Bank, USA Cat# DSHB 8C11, RRID: AB_528083 Mouse anti-Wg Developmental State Hybridoma Bank, USA Cat# DSHB 4D4, RRID:AB_528512 Rat anti-Ci Developmental State Hybridoma Bank, USA Cat# DSHB 2A1, RRID:AB_2109711 Rabbit anti-pMad Abcam Cat# ab52903, RRID:AB_882596 Mouse anti-phosphoCaMKII ThermoFisher Scientific Cat# MA1-047, RRID: AB_325402 Mouse anti-P1 Gift from Dr. Istvan Ando, Hungarian Academy of Sciences, Hungary N/A
Article Title: Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling
Article Snippet: Primary antibodies were used for staining were: Mouse anti-V5 (Invitrogen R960-25; preabsorbed at 1:10 dilution, then used at 1:50), Rat anti-E-cadherin (DSHB DCAD2-c 1:200), Chicken anti-ß-gal (Abcam ab9361; 1:100), Rat anti-Fat (1:1500) , Mouse anti-Wg (DSHB 9A4; 1:300), Rabbit anti-Dcr2 (Abcam ab4732, 1:1600), Rabbit anti-FLAG (Sigma F3165; 1:200).
Article Title: InR and Pi3K maintain intestinal homeostasis through STAT/EGFR and Notch signaling in enteroblasts.
Article Snippet: Funding information Fundamental Research Funds for the Central Universities, Grant/Award Number: 2572022DQ07; National Natural Science Foundation of China, Grant/Award Number: 32170484 Abstract To maintain the integrity of the adult gut, the proliferation and differentiation of stem cells must be strictly controlled.. Several signaling pathways control the proliferation and differentiation of Drosophila intestinal epithelial cells.. Although the modulatory effects of insulin pathway components on cell proliferation have been characterized, their specific role in which cell type and how these components interact with other regulatory signaling pathways remain largely unclear.
Electron Microscopy:Article Title: Nuclear receptor E75/NR1D2 drives tumor malignant transformation by integrating Hippo and Notch pathways
Article Snippet: Primary antibodies used include mouse Anti-beta Galactosidase (1:100, DSHB), mouse anti-Br (1:100, DSHB), rat anti- Elav (1:100, DSHB), mouse anti-Mmp-1 (1:100, DSHB), mouse anti-wg (1:100, DSHB), Cyclin E (1:100, Santa Cruz), rabbit anti-Dcp1 (CST, 1:100), mouse anti-dMyc (1:50, DSHB), mouse anti-Cut (1:100, DSHB), mouse anti-NICD (1:100, DSHB).
Article Title: Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila.
Article Snippet: This article is protected by copyright.. All rights reserved.. A cc ep te d A rt ic le Ashim Mukherjee ORCID iD: 0000-0002-3523-7089 Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila Vartika Sharma, Mousumi Mutsuddi, Ashim Mukherjee* Department of Molecular and Human Genetics, Institute of Science, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India *Corresponding Author Email: amukherjee@bhu.ac.in
Article Title: Microvilli-derived extracellular vesicles carry Hedgehog morphogenic signals for Drosophila wing imaginal disc development.
Article Snippet: Antibodies were used as follows: mouse anti-En (1:1000, Development Studies Hybridoma Bank University of Iowa – DSHB), rabbit anti-En (1:1000, Santa Cruz), mouse anti-Ptc42 (1/400), chicken anti-bgal (1:1000, Gen Tex), affinity-purified rabbit ‘‘Calvados’’ polyclonal anti-Hh43 (1:200), mouse anti-Cut (1:100, DSHB), mouse anti-Wg (1:50, DSHB), guinea pig anti-PromL (1:150, this study), mouse anti Dll44 (1:100), rabbit anti-aPKC (1:500, Santa Cruz), rat anti-DE-cadherin (1:50, DSHB), mouse anti-Dlg (1:100, DSHB);mouse anti-Arm (1:100,DSHB),mouse anti-GFP (1:1000, Roche), rabbit anti-Cad99c25 (1:1000, D. Godt, University of Toronto), rabbit anti-Caspase3 (1:500,CellSignaling).
Article Title: Body-fat sensor triggers ribosome maturation in the steroidogenic gland to initiate sexual maturation in Drosophila.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-Dlg DSHB Cat#4F3; RRID: AB_528203 mouse anti-Fibrillarin ThermoFisher Scientific Cat#38F3; RRID: AB_2532241 mouse anti-Lam Dm0 DSHB Cat#ADL84.12; RRID:AB_528338 mouse anti-Wg DSHB Cat#4D4; RRID: AB_528512 rabbit anti-Apolpp Joaquin Culi, Kutty et al., 1996 N/A rabbit anti-GFP Abcam Cat#Ab290 rabbit anti-IgG HRP-conjugated Sigma-Aldrich Cat# A9169 rabbit anti-ILP2 Hugo Stocker, Bader et al., 2013 N/A rabbit anti-Sema1a Alex Kolodkin, Yu et al., 1998 N/A rat anti-DE-Cad DSHB Cat#DCAD2; RRID: AB_528120 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31570; RRID: AB_2536180 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31572; RRID: AB_162543 donkey Anti-Rat IgG (H+L) Alexa Fluor 647 AffiniPure Jackson ImmunoResearch Cat#712-605-153; RRID: AB_2340694 Chemicals, peptides, and recombinant proteins Vectashield mounting medium with DAPI Vector Labs Cat#H-1200 DAPI-Fluoromount-G Electron Microscopy Sciences Cat#17984-24 GIBCO Schneider’s Drosophila Sterile Medium Thermo Fisher Cat#21720024 Bromophenol blue Sigma-Aldrich Cat#B6131 a-ecdysone Sigma-Aldrich Cat#9004 20-hydroxyecdysone Sigma-Aldrich Cat#H5142 SuperScript III First-Strand Synthesis System for RT-PCR Invitrogen Cat#18080-051 Power SYBR Green PCR Master Mix Applied Biosystems Cat#4367659 Paraformaldehyde 16% solution Electron Microscopy Sciences Cat#15710 Nile Red Sigma-Aldrich Cat#72485 1X cOmplete Mini EDTAfree protease inhibitor cocktail Sigma-Aldrich Cat#11836170001 Phalloidin 633 ThermoFisher Cat#A22284 Critical commercial assays 20-Hydroxyecdysone enzyme immunoassay kit Bertin Pharma Cat#A05120 Amplex Red Cholesterol Assay Kit ThermoFisher Scientific Cat#A12216 BCA Protein Assay Kit Pierce Cat#23227 Glucose (HK) Assay Kit Sigma-Aldrich Cat#GAHK20-1KT RNeasy-Mini Kit QIAGEN Cat#74106 RNase Free DNase Set QIAGEN Cat#79254 Experimental models: Organisms/strains D. melanogaster: mir-8NP5247-Gal4 DGRC 104917 D. melanogaster: NP2222-Gal4 DRGC 112830 D. melanogaster: phm22-Gal4 Kim Rewitz, Ono et al., 2006 N/A (Continued on next page) Cell Reports 37, 109830, October 12, 2021 e1
Article Title:
Article Snippet: Mouse anti-Antennapedia Developmental State Hybridoma Bank, USA Cat# DSHB 8C11, RRID: AB_528083 Mouse anti-Wg Developmental State Hybridoma Bank, USA Cat# DSHB 4D4, RRID:AB_528512 Rat anti-Ci Developmental State Hybridoma Bank, USA Cat# DSHB 2A1, RRID:AB_2109711 Rabbit anti-pMad Abcam Cat# ab52903, RRID:AB_882596 Mouse anti-phosphoCaMKII ThermoFisher Scientific Cat# MA1-047, RRID: AB_325402 Mouse anti-P1 Gift from Dr. Istvan Ando, Hungarian Academy of Sciences, Hungary N/A
Article Title: Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling
Article Snippet: Primary antibodies were used for staining were: Mouse anti-V5 (Invitrogen R960-25; preabsorbed at 1:10 dilution, then used at 1:50), Rat anti-E-cadherin (DSHB DCAD2-c 1:200), Chicken anti-ß-gal (Abcam ab9361; 1:100), Rat anti-Fat (1:1500) , Mouse anti-Wg (DSHB 9A4; 1:300), Rabbit anti-Dcr2 (Abcam ab4732, 1:1600), Rabbit anti-FLAG (Sigma F3165; 1:200).
Article Title: InR and Pi3K maintain intestinal homeostasis through STAT/EGFR and Notch signaling in enteroblasts.
Article Snippet: Funding information Fundamental Research Funds for the Central Universities, Grant/Award Number: 2572022DQ07; National Natural Science Foundation of China, Grant/Award Number: 32170484 Abstract To maintain the integrity of the adult gut, the proliferation and differentiation of stem cells must be strictly controlled.. Several signaling pathways control the proliferation and differentiation of Drosophila intestinal epithelial cells.. Although the modulatory effects of insulin pathway components on cell proliferation have been characterized, their specific role in which cell type and how these components interact with other regulatory signaling pathways remain largely unclear.
Sterility:Article Title: Nuclear receptor E75/NR1D2 drives tumor malignant transformation by integrating Hippo and Notch pathways
Article Snippet: Primary antibodies used include mouse Anti-beta Galactosidase (1:100, DSHB), mouse anti-Br (1:100, DSHB), rat anti- Elav (1:100, DSHB), mouse anti-Mmp-1 (1:100, DSHB), mouse anti-wg (1:100, DSHB), Cyclin E (1:100, Santa Cruz), rabbit anti-Dcp1 (CST, 1:100), mouse anti-dMyc (1:50, DSHB), mouse anti-Cut (1:100, DSHB), mouse anti-NICD (1:100, DSHB).
Article Title: Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila.
Article Snippet: This article is protected by copyright.. All rights reserved.. A cc ep te d A rt ic le Ashim Mukherjee ORCID iD: 0000-0002-3523-7089 Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila Vartika Sharma, Mousumi Mutsuddi, Ashim Mukherjee* Department of Molecular and Human Genetics, Institute of Science, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India *Corresponding Author Email: amukherjee@bhu.ac.in
Article Title: Microvilli-derived extracellular vesicles carry Hedgehog morphogenic signals for Drosophila wing imaginal disc development.
Article Snippet: Antibodies were used as follows: mouse anti-En (1:1000, Development Studies Hybridoma Bank University of Iowa – DSHB), rabbit anti-En (1:1000, Santa Cruz), mouse anti-Ptc42 (1/400), chicken anti-bgal (1:1000, Gen Tex), affinity-purified rabbit ‘‘Calvados’’ polyclonal anti-Hh43 (1:200), mouse anti-Cut (1:100, DSHB), mouse anti-Wg (1:50, DSHB), guinea pig anti-PromL (1:150, this study), mouse anti Dll44 (1:100), rabbit anti-aPKC (1:500, Santa Cruz), rat anti-DE-cadherin (1:50, DSHB), mouse anti-Dlg (1:100, DSHB);mouse anti-Arm (1:100,DSHB),mouse anti-GFP (1:1000, Roche), rabbit anti-Cad99c25 (1:1000, D. Godt, University of Toronto), rabbit anti-Caspase3 (1:500,CellSignaling).
Article Title: Body-fat sensor triggers ribosome maturation in the steroidogenic gland to initiate sexual maturation in Drosophila.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-Dlg DSHB Cat#4F3; RRID: AB_528203 mouse anti-Fibrillarin ThermoFisher Scientific Cat#38F3; RRID: AB_2532241 mouse anti-Lam Dm0 DSHB Cat#ADL84.12; RRID:AB_528338 mouse anti-Wg DSHB Cat#4D4; RRID: AB_528512 rabbit anti-Apolpp Joaquin Culi, Kutty et al., 1996 N/A rabbit anti-GFP Abcam Cat#Ab290 rabbit anti-IgG HRP-conjugated Sigma-Aldrich Cat# A9169 rabbit anti-ILP2 Hugo Stocker, Bader et al., 2013 N/A rabbit anti-Sema1a Alex Kolodkin, Yu et al., 1998 N/A rat anti-DE-Cad DSHB Cat#DCAD2; RRID: AB_528120 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31570; RRID: AB_2536180 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31572; RRID: AB_162543 donkey Anti-Rat IgG (H+L) Alexa Fluor 647 AffiniPure Jackson ImmunoResearch Cat#712-605-153; RRID: AB_2340694 Chemicals, peptides, and recombinant proteins Vectashield mounting medium with DAPI Vector Labs Cat#H-1200 DAPI-Fluoromount-G Electron Microscopy Sciences Cat#17984-24 GIBCO Schneider’s Drosophila Sterile Medium Thermo Fisher Cat#21720024 Bromophenol blue Sigma-Aldrich Cat#B6131 a-ecdysone Sigma-Aldrich Cat#9004 20-hydroxyecdysone Sigma-Aldrich Cat#H5142 SuperScript III First-Strand Synthesis System for RT-PCR Invitrogen Cat#18080-051 Power SYBR Green PCR Master Mix Applied Biosystems Cat#4367659 Paraformaldehyde 16% solution Electron Microscopy Sciences Cat#15710 Nile Red Sigma-Aldrich Cat#72485 1X cOmplete Mini EDTAfree protease inhibitor cocktail Sigma-Aldrich Cat#11836170001 Phalloidin 633 ThermoFisher Cat#A22284 Critical commercial assays 20-Hydroxyecdysone enzyme immunoassay kit Bertin Pharma Cat#A05120 Amplex Red Cholesterol Assay Kit ThermoFisher Scientific Cat#A12216 BCA Protein Assay Kit Pierce Cat#23227 Glucose (HK) Assay Kit Sigma-Aldrich Cat#GAHK20-1KT RNeasy-Mini Kit QIAGEN Cat#74106 RNase Free DNase Set QIAGEN Cat#79254 Experimental models: Organisms/strains D. melanogaster: mir-8NP5247-Gal4 DGRC 104917 D. melanogaster: NP2222-Gal4 DRGC 112830 D. melanogaster: phm22-Gal4 Kim Rewitz, Ono et al., 2006 N/A (Continued on next page) Cell Reports 37, 109830, October 12, 2021 e1
Article Title:
Article Snippet: Mouse anti-Antennapedia Developmental State Hybridoma Bank, USA Cat# DSHB 8C11, RRID: AB_528083 Mouse anti-Wg Developmental State Hybridoma Bank, USA Cat# DSHB 4D4, RRID:AB_528512 Rat anti-Ci Developmental State Hybridoma Bank, USA Cat# DSHB 2A1, RRID:AB_2109711 Rabbit anti-pMad Abcam Cat# ab52903, RRID:AB_882596 Mouse anti-phosphoCaMKII ThermoFisher Scientific Cat# MA1-047, RRID: AB_325402 Mouse anti-P1 Gift from Dr. Istvan Ando, Hungarian Academy of Sciences, Hungary N/A
Article Title: Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling
Article Snippet: Primary antibodies were used for staining were: Mouse anti-V5 (Invitrogen R960-25; preabsorbed at 1:10 dilution, then used at 1:50), Rat anti-E-cadherin (DSHB DCAD2-c 1:200), Chicken anti-ß-gal (Abcam ab9361; 1:100), Rat anti-Fat (1:1500) , Mouse anti-Wg (DSHB 9A4; 1:300), Rabbit anti-Dcr2 (Abcam ab4732, 1:1600), Rabbit anti-FLAG (Sigma F3165; 1:200).
Article Title: InR and Pi3K maintain intestinal homeostasis through STAT/EGFR and Notch signaling in enteroblasts.
Article Snippet: Funding information Fundamental Research Funds for the Central Universities, Grant/Award Number: 2572022DQ07; National Natural Science Foundation of China, Grant/Award Number: 32170484 Abstract To maintain the integrity of the adult gut, the proliferation and differentiation of stem cells must be strictly controlled.. Several signaling pathways control the proliferation and differentiation of Drosophila intestinal epithelial cells.. Although the modulatory effects of insulin pathway components on cell proliferation have been characterized, their specific role in which cell type and how these components interact with other regulatory signaling pathways remain largely unclear.
Reverse Transcription Polymerase Chain Reaction:Article Title: Nuclear receptor E75/NR1D2 drives tumor malignant transformation by integrating Hippo and Notch pathways
Article Snippet: Primary antibodies used include mouse Anti-beta Galactosidase (1:100, DSHB), mouse anti-Br (1:100, DSHB), rat anti- Elav (1:100, DSHB), mouse anti-Mmp-1 (1:100, DSHB), mouse anti-wg (1:100, DSHB), Cyclin E (1:100, Santa Cruz), rabbit anti-Dcp1 (CST, 1:100), mouse anti-dMyc (1:50, DSHB), mouse anti-Cut (1:100, DSHB), mouse anti-NICD (1:100, DSHB).
Article Title: Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila.
Article Snippet: This article is protected by copyright.. All rights reserved.. A cc ep te d A rt ic le Ashim Mukherjee ORCID iD: 0000-0002-3523-7089 Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila Vartika Sharma, Mousumi Mutsuddi, Ashim Mukherjee* Department of Molecular and Human Genetics, Institute of Science, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India *Corresponding Author Email: amukherjee@bhu.ac.in
Article Title: Microvilli-derived extracellular vesicles carry Hedgehog morphogenic signals for Drosophila wing imaginal disc development.
Article Snippet: Antibodies were used as follows: mouse anti-En (1:1000, Development Studies Hybridoma Bank University of Iowa – DSHB), rabbit anti-En (1:1000, Santa Cruz), mouse anti-Ptc42 (1/400), chicken anti-bgal (1:1000, Gen Tex), affinity-purified rabbit ‘‘Calvados’’ polyclonal anti-Hh43 (1:200), mouse anti-Cut (1:100, DSHB), mouse anti-Wg (1:50, DSHB), guinea pig anti-PromL (1:150, this study), mouse anti Dll44 (1:100), rabbit anti-aPKC (1:500, Santa Cruz), rat anti-DE-cadherin (1:50, DSHB), mouse anti-Dlg (1:100, DSHB);mouse anti-Arm (1:100,DSHB),mouse anti-GFP (1:1000, Roche), rabbit anti-Cad99c25 (1:1000, D. Godt, University of Toronto), rabbit anti-Caspase3 (1:500,CellSignaling).
Article Title: Body-fat sensor triggers ribosome maturation in the steroidogenic gland to initiate sexual maturation in Drosophila.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-Dlg DSHB Cat#4F3; RRID: AB_528203 mouse anti-Fibrillarin ThermoFisher Scientific Cat#38F3; RRID: AB_2532241 mouse anti-Lam Dm0 DSHB Cat#ADL84.12; RRID:AB_528338 mouse anti-Wg DSHB Cat#4D4; RRID: AB_528512 rabbit anti-Apolpp Joaquin Culi, Kutty et al., 1996 N/A rabbit anti-GFP Abcam Cat#Ab290 rabbit anti-IgG HRP-conjugated Sigma-Aldrich Cat# A9169 rabbit anti-ILP2 Hugo Stocker, Bader et al., 2013 N/A rabbit anti-Sema1a Alex Kolodkin, Yu et al., 1998 N/A rat anti-DE-Cad DSHB Cat#DCAD2; RRID: AB_528120 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31570; RRID: AB_2536180 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31572; RRID: AB_162543 donkey Anti-Rat IgG (H+L) Alexa Fluor 647 AffiniPure Jackson ImmunoResearch Cat#712-605-153; RRID: AB_2340694 Chemicals, peptides, and recombinant proteins Vectashield mounting medium with DAPI Vector Labs Cat#H-1200 DAPI-Fluoromount-G Electron Microscopy Sciences Cat#17984-24 GIBCO Schneider’s Drosophila Sterile Medium Thermo Fisher Cat#21720024 Bromophenol blue Sigma-Aldrich Cat#B6131 a-ecdysone Sigma-Aldrich Cat#9004 20-hydroxyecdysone Sigma-Aldrich Cat#H5142 SuperScript III First-Strand Synthesis System for RT-PCR Invitrogen Cat#18080-051 Power SYBR Green PCR Master Mix Applied Biosystems Cat#4367659 Paraformaldehyde 16% solution Electron Microscopy Sciences Cat#15710 Nile Red Sigma-Aldrich Cat#72485 1X cOmplete Mini EDTAfree protease inhibitor cocktail Sigma-Aldrich Cat#11836170001 Phalloidin 633 ThermoFisher Cat#A22284 Critical commercial assays 20-Hydroxyecdysone enzyme immunoassay kit Bertin Pharma Cat#A05120 Amplex Red Cholesterol Assay Kit ThermoFisher Scientific Cat#A12216 BCA Protein Assay Kit Pierce Cat#23227 Glucose (HK) Assay Kit Sigma-Aldrich Cat#GAHK20-1KT RNeasy-Mini Kit QIAGEN Cat#74106 RNase Free DNase Set QIAGEN Cat#79254 Experimental models: Organisms/strains D. melanogaster: mir-8NP5247-Gal4 DGRC 104917 D. melanogaster: NP2222-Gal4 DRGC 112830 D. melanogaster: phm22-Gal4 Kim Rewitz, Ono et al., 2006 N/A (Continued on next page) Cell Reports 37, 109830, October 12, 2021 e1
Article Title:
Article Snippet: Mouse anti-Antennapedia Developmental State Hybridoma Bank, USA Cat# DSHB 8C11, RRID: AB_528083 Mouse anti-Wg Developmental State Hybridoma Bank, USA Cat# DSHB 4D4, RRID:AB_528512 Rat anti-Ci Developmental State Hybridoma Bank, USA Cat# DSHB 2A1, RRID:AB_2109711 Rabbit anti-pMad Abcam Cat# ab52903, RRID:AB_882596 Mouse anti-phosphoCaMKII ThermoFisher Scientific Cat# MA1-047, RRID: AB_325402 Mouse anti-P1 Gift from Dr. Istvan Ando, Hungarian Academy of Sciences, Hungary N/A
Article Title: Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling
Article Snippet: Primary antibodies were used for staining were: Mouse anti-V5 (Invitrogen R960-25; preabsorbed at 1:10 dilution, then used at 1:50), Rat anti-E-cadherin (DSHB DCAD2-c 1:200), Chicken anti-ß-gal (Abcam ab9361; 1:100), Rat anti-Fat (1:1500) , Mouse anti-Wg (DSHB 9A4; 1:300), Rabbit anti-Dcr2 (Abcam ab4732, 1:1600), Rabbit anti-FLAG (Sigma F3165; 1:200).
Article Title: InR and Pi3K maintain intestinal homeostasis through STAT/EGFR and Notch signaling in enteroblasts.
Article Snippet: Funding information Fundamental Research Funds for the Central Universities, Grant/Award Number: 2572022DQ07; National Natural Science Foundation of China, Grant/Award Number: 32170484 Abstract To maintain the integrity of the adult gut, the proliferation and differentiation of stem cells must be strictly controlled.. Several signaling pathways control the proliferation and differentiation of Drosophila intestinal epithelial cells.. Although the modulatory effects of insulin pathway components on cell proliferation have been characterized, their specific role in which cell type and how these components interact with other regulatory signaling pathways remain largely unclear.
SYBR Green Assay:Article Title: Nuclear receptor E75/NR1D2 drives tumor malignant transformation by integrating Hippo and Notch pathways
Article Snippet: Primary antibodies used include mouse Anti-beta Galactosidase (1:100, DSHB), mouse anti-Br (1:100, DSHB), rat anti- Elav (1:100, DSHB), mouse anti-Mmp-1 (1:100, DSHB), mouse anti-wg (1:100, DSHB), Cyclin E (1:100, Santa Cruz), rabbit anti-Dcp1 (CST, 1:100), mouse anti-dMyc (1:50, DSHB), mouse anti-Cut (1:100, DSHB), mouse anti-NICD (1:100, DSHB).
Article Title: Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila.
Article Snippet: This article is protected by copyright.. All rights reserved.. A cc ep te d A rt ic le Ashim Mukherjee ORCID iD: 0000-0002-3523-7089 Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila Vartika Sharma, Mousumi Mutsuddi, Ashim Mukherjee* Department of Molecular and Human Genetics, Institute of Science, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India *Corresponding Author Email: amukherjee@bhu.ac.in
Article Title: Microvilli-derived extracellular vesicles carry Hedgehog morphogenic signals for Drosophila wing imaginal disc development.
Article Snippet: Antibodies were used as follows: mouse anti-En (1:1000, Development Studies Hybridoma Bank University of Iowa – DSHB), rabbit anti-En (1:1000, Santa Cruz), mouse anti-Ptc42 (1/400), chicken anti-bgal (1:1000, Gen Tex), affinity-purified rabbit ‘‘Calvados’’ polyclonal anti-Hh43 (1:200), mouse anti-Cut (1:100, DSHB), mouse anti-Wg (1:50, DSHB), guinea pig anti-PromL (1:150, this study), mouse anti Dll44 (1:100), rabbit anti-aPKC (1:500, Santa Cruz), rat anti-DE-cadherin (1:50, DSHB), mouse anti-Dlg (1:100, DSHB);mouse anti-Arm (1:100,DSHB),mouse anti-GFP (1:1000, Roche), rabbit anti-Cad99c25 (1:1000, D. Godt, University of Toronto), rabbit anti-Caspase3 (1:500,CellSignaling).
Article Title: Body-fat sensor triggers ribosome maturation in the steroidogenic gland to initiate sexual maturation in Drosophila.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-Dlg DSHB Cat#4F3; RRID: AB_528203 mouse anti-Fibrillarin ThermoFisher Scientific Cat#38F3; RRID: AB_2532241 mouse anti-Lam Dm0 DSHB Cat#ADL84.12; RRID:AB_528338 mouse anti-Wg DSHB Cat#4D4; RRID: AB_528512 rabbit anti-Apolpp Joaquin Culi, Kutty et al., 1996 N/A rabbit anti-GFP Abcam Cat#Ab290 rabbit anti-IgG HRP-conjugated Sigma-Aldrich Cat# A9169 rabbit anti-ILP2 Hugo Stocker, Bader et al., 2013 N/A rabbit anti-Sema1a Alex Kolodkin, Yu et al., 1998 N/A rat anti-DE-Cad DSHB Cat#DCAD2; RRID: AB_528120 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31570; RRID: AB_2536180 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31572; RRID: AB_162543 donkey Anti-Rat IgG (H+L) Alexa Fluor 647 AffiniPure Jackson ImmunoResearch Cat#712-605-153; RRID: AB_2340694 Chemicals, peptides, and recombinant proteins Vectashield mounting medium with DAPI Vector Labs Cat#H-1200 DAPI-Fluoromount-G Electron Microscopy Sciences Cat#17984-24 GIBCO Schneider’s Drosophila Sterile Medium Thermo Fisher Cat#21720024 Bromophenol blue Sigma-Aldrich Cat#B6131 a-ecdysone Sigma-Aldrich Cat#9004 20-hydroxyecdysone Sigma-Aldrich Cat#H5142 SuperScript III First-Strand Synthesis System for RT-PCR Invitrogen Cat#18080-051 Power SYBR Green PCR Master Mix Applied Biosystems Cat#4367659 Paraformaldehyde 16% solution Electron Microscopy Sciences Cat#15710 Nile Red Sigma-Aldrich Cat#72485 1X cOmplete Mini EDTAfree protease inhibitor cocktail Sigma-Aldrich Cat#11836170001 Phalloidin 633 ThermoFisher Cat#A22284 Critical commercial assays 20-Hydroxyecdysone enzyme immunoassay kit Bertin Pharma Cat#A05120 Amplex Red Cholesterol Assay Kit ThermoFisher Scientific Cat#A12216 BCA Protein Assay Kit Pierce Cat#23227 Glucose (HK) Assay Kit Sigma-Aldrich Cat#GAHK20-1KT RNeasy-Mini Kit QIAGEN Cat#74106 RNase Free DNase Set QIAGEN Cat#79254 Experimental models: Organisms/strains D. melanogaster: mir-8NP5247-Gal4 DGRC 104917 D. melanogaster: NP2222-Gal4 DRGC 112830 D. melanogaster: phm22-Gal4 Kim Rewitz, Ono et al., 2006 N/A (Continued on next page) Cell Reports 37, 109830, October 12, 2021 e1
Article Title:
Article Snippet: Mouse anti-Antennapedia Developmental State Hybridoma Bank, USA Cat# DSHB 8C11, RRID: AB_528083 Mouse anti-Wg Developmental State Hybridoma Bank, USA Cat# DSHB 4D4, RRID:AB_528512 Rat anti-Ci Developmental State Hybridoma Bank, USA Cat# DSHB 2A1, RRID:AB_2109711 Rabbit anti-pMad Abcam Cat# ab52903, RRID:AB_882596 Mouse anti-phosphoCaMKII ThermoFisher Scientific Cat# MA1-047, RRID: AB_325402 Mouse anti-P1 Gift from Dr. Istvan Ando, Hungarian Academy of Sciences, Hungary N/A
Article Title: Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling
Article Snippet: Primary antibodies were used for staining were: Mouse anti-V5 (Invitrogen R960-25; preabsorbed at 1:10 dilution, then used at 1:50), Rat anti-E-cadherin (DSHB DCAD2-c 1:200), Chicken anti-ß-gal (Abcam ab9361; 1:100), Rat anti-Fat (1:1500) , Mouse anti-Wg (DSHB 9A4; 1:300), Rabbit anti-Dcr2 (Abcam ab4732, 1:1600), Rabbit anti-FLAG (Sigma F3165; 1:200).
Article Title: InR and Pi3K maintain intestinal homeostasis through STAT/EGFR and Notch signaling in enteroblasts.
Article Snippet: Funding information Fundamental Research Funds for the Central Universities, Grant/Award Number: 2572022DQ07; National Natural Science Foundation of China, Grant/Award Number: 32170484 Abstract To maintain the integrity of the adult gut, the proliferation and differentiation of stem cells must be strictly controlled.. Several signaling pathways control the proliferation and differentiation of Drosophila intestinal epithelial cells.. Although the modulatory effects of insulin pathway components on cell proliferation have been characterized, their specific role in which cell type and how these components interact with other regulatory signaling pathways remain largely unclear.
Polymerase Chain Reaction:Article Title: Nuclear receptor E75/NR1D2 drives tumor malignant transformation by integrating Hippo and Notch pathways
Article Snippet: Primary antibodies used include mouse Anti-beta Galactosidase (1:100, DSHB), mouse anti-Br (1:100, DSHB), rat anti- Elav (1:100, DSHB), mouse anti-Mmp-1 (1:100, DSHB), mouse anti-wg (1:100, DSHB), Cyclin E (1:100, Santa Cruz), rabbit anti-Dcp1 (CST, 1:100), mouse anti-dMyc (1:50, DSHB), mouse anti-Cut (1:100, DSHB), mouse anti-NICD (1:100, DSHB).
Article Title: Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila.
Article Snippet: This article is protected by copyright.. All rights reserved.. A cc ep te d A rt ic le Ashim Mukherjee ORCID iD: 0000-0002-3523-7089 Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila Vartika Sharma, Mousumi Mutsuddi, Ashim Mukherjee* Department of Molecular and Human Genetics, Institute of Science, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India *Corresponding Author Email: amukherjee@bhu.ac.in
Article Title: Microvilli-derived extracellular vesicles carry Hedgehog morphogenic signals for Drosophila wing imaginal disc development.
Article Snippet: Antibodies were used as follows: mouse anti-En (1:1000, Development Studies Hybridoma Bank University of Iowa – DSHB), rabbit anti-En (1:1000, Santa Cruz), mouse anti-Ptc42 (1/400), chicken anti-bgal (1:1000, Gen Tex), affinity-purified rabbit ‘‘Calvados’’ polyclonal anti-Hh43 (1:200), mouse anti-Cut (1:100, DSHB), mouse anti-Wg (1:50, DSHB), guinea pig anti-PromL (1:150, this study), mouse anti Dll44 (1:100), rabbit anti-aPKC (1:500, Santa Cruz), rat anti-DE-cadherin (1:50, DSHB), mouse anti-Dlg (1:100, DSHB);mouse anti-Arm (1:100,DSHB),mouse anti-GFP (1:1000, Roche), rabbit anti-Cad99c25 (1:1000, D. Godt, University of Toronto), rabbit anti-Caspase3 (1:500,CellSignaling).
Article Title: Body-fat sensor triggers ribosome maturation in the steroidogenic gland to initiate sexual maturation in Drosophila.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-Dlg DSHB Cat#4F3; RRID: AB_528203 mouse anti-Fibrillarin ThermoFisher Scientific Cat#38F3; RRID: AB_2532241 mouse anti-Lam Dm0 DSHB Cat#ADL84.12; RRID:AB_528338 mouse anti-Wg DSHB Cat#4D4; RRID: AB_528512 rabbit anti-Apolpp Joaquin Culi, Kutty et al., 1996 N/A rabbit anti-GFP Abcam Cat#Ab290 rabbit anti-IgG HRP-conjugated Sigma-Aldrich Cat# A9169 rabbit anti-ILP2 Hugo Stocker, Bader et al., 2013 N/A rabbit anti-Sema1a Alex Kolodkin, Yu et al., 1998 N/A rat anti-DE-Cad DSHB Cat#DCAD2; RRID: AB_528120 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31570; RRID: AB_2536180 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31572; RRID: AB_162543 donkey Anti-Rat IgG (H+L) Alexa Fluor 647 AffiniPure Jackson ImmunoResearch Cat#712-605-153; RRID: AB_2340694 Chemicals, peptides, and recombinant proteins Vectashield mounting medium with DAPI Vector Labs Cat#H-1200 DAPI-Fluoromount-G Electron Microscopy Sciences Cat#17984-24 GIBCO Schneider’s Drosophila Sterile Medium Thermo Fisher Cat#21720024 Bromophenol blue Sigma-Aldrich Cat#B6131 a-ecdysone Sigma-Aldrich Cat#9004 20-hydroxyecdysone Sigma-Aldrich Cat#H5142 SuperScript III First-Strand Synthesis System for RT-PCR Invitrogen Cat#18080-051 Power SYBR Green PCR Master Mix Applied Biosystems Cat#4367659 Paraformaldehyde 16% solution Electron Microscopy Sciences Cat#15710 Nile Red Sigma-Aldrich Cat#72485 1X cOmplete Mini EDTAfree protease inhibitor cocktail Sigma-Aldrich Cat#11836170001 Phalloidin 633 ThermoFisher Cat#A22284 Critical commercial assays 20-Hydroxyecdysone enzyme immunoassay kit Bertin Pharma Cat#A05120 Amplex Red Cholesterol Assay Kit ThermoFisher Scientific Cat#A12216 BCA Protein Assay Kit Pierce Cat#23227 Glucose (HK) Assay Kit Sigma-Aldrich Cat#GAHK20-1KT RNeasy-Mini Kit QIAGEN Cat#74106 RNase Free DNase Set QIAGEN Cat#79254 Experimental models: Organisms/strains D. melanogaster: mir-8NP5247-Gal4 DGRC 104917 D. melanogaster: NP2222-Gal4 DRGC 112830 D. melanogaster: phm22-Gal4 Kim Rewitz, Ono et al., 2006 N/A (Continued on next page) Cell Reports 37, 109830, October 12, 2021 e1
Article Title:
Article Snippet: Mouse anti-Antennapedia Developmental State Hybridoma Bank, USA Cat# DSHB 8C11, RRID: AB_528083 Mouse anti-Wg Developmental State Hybridoma Bank, USA Cat# DSHB 4D4, RRID:AB_528512 Rat anti-Ci Developmental State Hybridoma Bank, USA Cat# DSHB 2A1, RRID:AB_2109711 Rabbit anti-pMad Abcam Cat# ab52903, RRID:AB_882596 Mouse anti-phosphoCaMKII ThermoFisher Scientific Cat# MA1-047, RRID: AB_325402 Mouse anti-P1 Gift from Dr. Istvan Ando, Hungarian Academy of Sciences, Hungary N/A
Article Title: Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling
Article Snippet: Primary antibodies were used for staining were: Mouse anti-V5 (Invitrogen R960-25; preabsorbed at 1:10 dilution, then used at 1:50), Rat anti-E-cadherin (DSHB DCAD2-c 1:200), Chicken anti-ß-gal (Abcam ab9361; 1:100), Rat anti-Fat (1:1500) , Mouse anti-Wg (DSHB 9A4; 1:300), Rabbit anti-Dcr2 (Abcam ab4732, 1:1600), Rabbit anti-FLAG (Sigma F3165; 1:200).
Article Title: InR and Pi3K maintain intestinal homeostasis through STAT/EGFR and Notch signaling in enteroblasts.
Article Snippet: Funding information Fundamental Research Funds for the Central Universities, Grant/Award Number: 2572022DQ07; National Natural Science Foundation of China, Grant/Award Number: 32170484 Abstract To maintain the integrity of the adult gut, the proliferation and differentiation of stem cells must be strictly controlled.. Several signaling pathways control the proliferation and differentiation of Drosophila intestinal epithelial cells.. Although the modulatory effects of insulin pathway components on cell proliferation have been characterized, their specific role in which cell type and how these components interact with other regulatory signaling pathways remain largely unclear.
Protease Inhibitor:Article Title: Nuclear receptor E75/NR1D2 drives tumor malignant transformation by integrating Hippo and Notch pathways
Article Snippet: Primary antibodies used include mouse Anti-beta Galactosidase (1:100, DSHB), mouse anti-Br (1:100, DSHB), rat anti- Elav (1:100, DSHB), mouse anti-Mmp-1 (1:100, DSHB), mouse anti-wg (1:100, DSHB), Cyclin E (1:100, Santa Cruz), rabbit anti-Dcp1 (CST, 1:100), mouse anti-dMyc (1:50, DSHB), mouse anti-Cut (1:100, DSHB), mouse anti-NICD (1:100, DSHB).
Article Title: Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila.
Article Snippet: This article is protected by copyright.. All rights reserved.. A cc ep te d A rt ic le Ashim Mukherjee ORCID iD: 0000-0002-3523-7089 Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila Vartika Sharma, Mousumi Mutsuddi, Ashim Mukherjee* Department of Molecular and Human Genetics, Institute of Science, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India *Corresponding Author Email: amukherjee@bhu.ac.in
Article Title: Microvilli-derived extracellular vesicles carry Hedgehog morphogenic signals for Drosophila wing imaginal disc development.
Article Snippet: Antibodies were used as follows: mouse anti-En (1:1000, Development Studies Hybridoma Bank University of Iowa – DSHB), rabbit anti-En (1:1000, Santa Cruz), mouse anti-Ptc42 (1/400), chicken anti-bgal (1:1000, Gen Tex), affinity-purified rabbit ‘‘Calvados’’ polyclonal anti-Hh43 (1:200), mouse anti-Cut (1:100, DSHB), mouse anti-Wg (1:50, DSHB), guinea pig anti-PromL (1:150, this study), mouse anti Dll44 (1:100), rabbit anti-aPKC (1:500, Santa Cruz), rat anti-DE-cadherin (1:50, DSHB), mouse anti-Dlg (1:100, DSHB);mouse anti-Arm (1:100,DSHB),mouse anti-GFP (1:1000, Roche), rabbit anti-Cad99c25 (1:1000, D. Godt, University of Toronto), rabbit anti-Caspase3 (1:500,CellSignaling).
Article Title: Body-fat sensor triggers ribosome maturation in the steroidogenic gland to initiate sexual maturation in Drosophila.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-Dlg DSHB Cat#4F3; RRID: AB_528203 mouse anti-Fibrillarin ThermoFisher Scientific Cat#38F3; RRID: AB_2532241 mouse anti-Lam Dm0 DSHB Cat#ADL84.12; RRID:AB_528338 mouse anti-Wg DSHB Cat#4D4; RRID: AB_528512 rabbit anti-Apolpp Joaquin Culi, Kutty et al., 1996 N/A rabbit anti-GFP Abcam Cat#Ab290 rabbit anti-IgG HRP-conjugated Sigma-Aldrich Cat# A9169 rabbit anti-ILP2 Hugo Stocker, Bader et al., 2013 N/A rabbit anti-Sema1a Alex Kolodkin, Yu et al., 1998 N/A rat anti-DE-Cad DSHB Cat#DCAD2; RRID: AB_528120 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31570; RRID: AB_2536180 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31572; RRID: AB_162543 donkey Anti-Rat IgG (H+L) Alexa Fluor 647 AffiniPure Jackson ImmunoResearch Cat#712-605-153; RRID: AB_2340694 Chemicals, peptides, and recombinant proteins Vectashield mounting medium with DAPI Vector Labs Cat#H-1200 DAPI-Fluoromount-G Electron Microscopy Sciences Cat#17984-24 GIBCO Schneider’s Drosophila Sterile Medium Thermo Fisher Cat#21720024 Bromophenol blue Sigma-Aldrich Cat#B6131 a-ecdysone Sigma-Aldrich Cat#9004 20-hydroxyecdysone Sigma-Aldrich Cat#H5142 SuperScript III First-Strand Synthesis System for RT-PCR Invitrogen Cat#18080-051 Power SYBR Green PCR Master Mix Applied Biosystems Cat#4367659 Paraformaldehyde 16% solution Electron Microscopy Sciences Cat#15710 Nile Red Sigma-Aldrich Cat#72485 1X cOmplete Mini EDTAfree protease inhibitor cocktail Sigma-Aldrich Cat#11836170001 Phalloidin 633 ThermoFisher Cat#A22284 Critical commercial assays 20-Hydroxyecdysone enzyme immunoassay kit Bertin Pharma Cat#A05120 Amplex Red Cholesterol Assay Kit ThermoFisher Scientific Cat#A12216 BCA Protein Assay Kit Pierce Cat#23227 Glucose (HK) Assay Kit Sigma-Aldrich Cat#GAHK20-1KT RNeasy-Mini Kit QIAGEN Cat#74106 RNase Free DNase Set QIAGEN Cat#79254 Experimental models: Organisms/strains D. melanogaster: mir-8NP5247-Gal4 DGRC 104917 D. melanogaster: NP2222-Gal4 DRGC 112830 D. melanogaster: phm22-Gal4 Kim Rewitz, Ono et al., 2006 N/A (Continued on next page) Cell Reports 37, 109830, October 12, 2021 e1
Article Title:
Article Snippet: Mouse anti-Antennapedia Developmental State Hybridoma Bank, USA Cat# DSHB 8C11, RRID: AB_528083 Mouse anti-Wg Developmental State Hybridoma Bank, USA Cat# DSHB 4D4, RRID:AB_528512 Rat anti-Ci Developmental State Hybridoma Bank, USA Cat# DSHB 2A1, RRID:AB_2109711 Rabbit anti-pMad Abcam Cat# ab52903, RRID:AB_882596 Mouse anti-phosphoCaMKII ThermoFisher Scientific Cat# MA1-047, RRID: AB_325402 Mouse anti-P1 Gift from Dr. Istvan Ando, Hungarian Academy of Sciences, Hungary N/A
Article Title: Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling
Article Snippet: Primary antibodies were used for staining were: Mouse anti-V5 (Invitrogen R960-25; preabsorbed at 1:10 dilution, then used at 1:50), Rat anti-E-cadherin (DSHB DCAD2-c 1:200), Chicken anti-ß-gal (Abcam ab9361; 1:100), Rat anti-Fat (1:1500) , Mouse anti-Wg (DSHB 9A4; 1:300), Rabbit anti-Dcr2 (Abcam ab4732, 1:1600), Rabbit anti-FLAG (Sigma F3165; 1:200).
Article Title: InR and Pi3K maintain intestinal homeostasis through STAT/EGFR and Notch signaling in enteroblasts.
Article Snippet: Funding information Fundamental Research Funds for the Central Universities, Grant/Award Number: 2572022DQ07; National Natural Science Foundation of China, Grant/Award Number: 32170484 Abstract To maintain the integrity of the adult gut, the proliferation and differentiation of stem cells must be strictly controlled.. Several signaling pathways control the proliferation and differentiation of Drosophila intestinal epithelial cells.. Although the modulatory effects of insulin pathway components on cell proliferation have been characterized, their specific role in which cell type and how these components interact with other regulatory signaling pathways remain largely unclear.
Enzyme-linked Immunosorbent Assay:Article Title: Nuclear receptor E75/NR1D2 drives tumor malignant transformation by integrating Hippo and Notch pathways
Article Snippet: Primary antibodies used include mouse Anti-beta Galactosidase (1:100, DSHB), mouse anti-Br (1:100, DSHB), rat anti- Elav (1:100, DSHB), mouse anti-Mmp-1 (1:100, DSHB), mouse anti-wg (1:100, DSHB), Cyclin E (1:100, Santa Cruz), rabbit anti-Dcp1 (CST, 1:100), mouse anti-dMyc (1:50, DSHB), mouse anti-Cut (1:100, DSHB), mouse anti-NICD (1:100, DSHB).
Article Title: Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila.
Article Snippet: This article is protected by copyright.. All rights reserved.. A cc ep te d A rt ic le Ashim Mukherjee ORCID iD: 0000-0002-3523-7089 Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila Vartika Sharma, Mousumi Mutsuddi, Ashim Mukherjee* Department of Molecular and Human Genetics, Institute of Science, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India *Corresponding Author Email: amukherjee@bhu.ac.in
Article Title: Microvilli-derived extracellular vesicles carry Hedgehog morphogenic signals for Drosophila wing imaginal disc development.
Article Snippet: Antibodies were used as follows: mouse anti-En (1:1000, Development Studies Hybridoma Bank University of Iowa – DSHB), rabbit anti-En (1:1000, Santa Cruz), mouse anti-Ptc42 (1/400), chicken anti-bgal (1:1000, Gen Tex), affinity-purified rabbit ‘‘Calvados’’ polyclonal anti-Hh43 (1:200), mouse anti-Cut (1:100, DSHB), mouse anti-Wg (1:50, DSHB), guinea pig anti-PromL (1:150, this study), mouse anti Dll44 (1:100), rabbit anti-aPKC (1:500, Santa Cruz), rat anti-DE-cadherin (1:50, DSHB), mouse anti-Dlg (1:100, DSHB);mouse anti-Arm (1:100,DSHB),mouse anti-GFP (1:1000, Roche), rabbit anti-Cad99c25 (1:1000, D. Godt, University of Toronto), rabbit anti-Caspase3 (1:500,CellSignaling).
Article Title: Body-fat sensor triggers ribosome maturation in the steroidogenic gland to initiate sexual maturation in Drosophila.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-Dlg DSHB Cat#4F3; RRID: AB_528203 mouse anti-Fibrillarin ThermoFisher Scientific Cat#38F3; RRID: AB_2532241 mouse anti-Lam Dm0 DSHB Cat#ADL84.12; RRID:AB_528338 mouse anti-Wg DSHB Cat#4D4; RRID: AB_528512 rabbit anti-Apolpp Joaquin Culi, Kutty et al., 1996 N/A rabbit anti-GFP Abcam Cat#Ab290 rabbit anti-IgG HRP-conjugated Sigma-Aldrich Cat# A9169 rabbit anti-ILP2 Hugo Stocker, Bader et al., 2013 N/A rabbit anti-Sema1a Alex Kolodkin, Yu et al., 1998 N/A rat anti-DE-Cad DSHB Cat#DCAD2; RRID: AB_528120 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31570; RRID: AB_2536180 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31572; RRID: AB_162543 donkey Anti-Rat IgG (H+L) Alexa Fluor 647 AffiniPure Jackson ImmunoResearch Cat#712-605-153; RRID: AB_2340694 Chemicals, peptides, and recombinant proteins Vectashield mounting medium with DAPI Vector Labs Cat#H-1200 DAPI-Fluoromount-G Electron Microscopy Sciences Cat#17984-24 GIBCO Schneider’s Drosophila Sterile Medium Thermo Fisher Cat#21720024 Bromophenol blue Sigma-Aldrich Cat#B6131 a-ecdysone Sigma-Aldrich Cat#9004 20-hydroxyecdysone Sigma-Aldrich Cat#H5142 SuperScript III First-Strand Synthesis System for RT-PCR Invitrogen Cat#18080-051 Power SYBR Green PCR Master Mix Applied Biosystems Cat#4367659 Paraformaldehyde 16% solution Electron Microscopy Sciences Cat#15710 Nile Red Sigma-Aldrich Cat#72485 1X cOmplete Mini EDTAfree protease inhibitor cocktail Sigma-Aldrich Cat#11836170001 Phalloidin 633 ThermoFisher Cat#A22284 Critical commercial assays 20-Hydroxyecdysone enzyme immunoassay kit Bertin Pharma Cat#A05120 Amplex Red Cholesterol Assay Kit ThermoFisher Scientific Cat#A12216 BCA Protein Assay Kit Pierce Cat#23227 Glucose (HK) Assay Kit Sigma-Aldrich Cat#GAHK20-1KT RNeasy-Mini Kit QIAGEN Cat#74106 RNase Free DNase Set QIAGEN Cat#79254 Experimental models: Organisms/strains D. melanogaster: mir-8NP5247-Gal4 DGRC 104917 D. melanogaster: NP2222-Gal4 DRGC 112830 D. melanogaster: phm22-Gal4 Kim Rewitz, Ono et al., 2006 N/A (Continued on next page) Cell Reports 37, 109830, October 12, 2021 e1
Article Title:
Article Snippet: Mouse anti-Antennapedia Developmental State Hybridoma Bank, USA Cat# DSHB 8C11, RRID: AB_528083 Mouse anti-Wg Developmental State Hybridoma Bank, USA Cat# DSHB 4D4, RRID:AB_528512 Rat anti-Ci Developmental State Hybridoma Bank, USA Cat# DSHB 2A1, RRID:AB_2109711 Rabbit anti-pMad Abcam Cat# ab52903, RRID:AB_882596 Mouse anti-phosphoCaMKII ThermoFisher Scientific Cat# MA1-047, RRID: AB_325402 Mouse anti-P1 Gift from Dr. Istvan Ando, Hungarian Academy of Sciences, Hungary N/A
Article Title: Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling
Article Snippet: Primary antibodies were used for staining were: Mouse anti-V5 (Invitrogen R960-25; preabsorbed at 1:10 dilution, then used at 1:50), Rat anti-E-cadherin (DSHB DCAD2-c 1:200), Chicken anti-ß-gal (Abcam ab9361; 1:100), Rat anti-Fat (1:1500) , Mouse anti-Wg (DSHB 9A4; 1:300), Rabbit anti-Dcr2 (Abcam ab4732, 1:1600), Rabbit anti-FLAG (Sigma F3165; 1:200).
Article Title: InR and Pi3K maintain intestinal homeostasis through STAT/EGFR and Notch signaling in enteroblasts.
Article Snippet: Funding information Fundamental Research Funds for the Central Universities, Grant/Award Number: 2572022DQ07; National Natural Science Foundation of China, Grant/Award Number: 32170484 Abstract To maintain the integrity of the adult gut, the proliferation and differentiation of stem cells must be strictly controlled.. Several signaling pathways control the proliferation and differentiation of Drosophila intestinal epithelial cells.. Although the modulatory effects of insulin pathway components on cell proliferation have been characterized, their specific role in which cell type and how these components interact with other regulatory signaling pathways remain largely unclear.
Amplex Red Cholesterol Assay:Article Title: Nuclear receptor E75/NR1D2 drives tumor malignant transformation by integrating Hippo and Notch pathways
Article Snippet: Primary antibodies used include mouse Anti-beta Galactosidase (1:100, DSHB), mouse anti-Br (1:100, DSHB), rat anti- Elav (1:100, DSHB), mouse anti-Mmp-1 (1:100, DSHB), mouse anti-wg (1:100, DSHB), Cyclin E (1:100, Santa Cruz), rabbit anti-Dcp1 (CST, 1:100), mouse anti-dMyc (1:50, DSHB), mouse anti-Cut (1:100, DSHB), mouse anti-NICD (1:100, DSHB).
Article Title: Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila.
Article Snippet: This article is protected by copyright.. All rights reserved.. A cc ep te d A rt ic le Ashim Mukherjee ORCID iD: 0000-0002-3523-7089 Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila Vartika Sharma, Mousumi Mutsuddi, Ashim Mukherjee* Department of Molecular and Human Genetics, Institute of Science, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India *Corresponding Author Email: amukherjee@bhu.ac.in
Article Title: Microvilli-derived extracellular vesicles carry Hedgehog morphogenic signals for Drosophila wing imaginal disc development.
Article Snippet: Antibodies were used as follows: mouse anti-En (1:1000, Development Studies Hybridoma Bank University of Iowa – DSHB), rabbit anti-En (1:1000, Santa Cruz), mouse anti-Ptc42 (1/400), chicken anti-bgal (1:1000, Gen Tex), affinity-purified rabbit ‘‘Calvados’’ polyclonal anti-Hh43 (1:200), mouse anti-Cut (1:100, DSHB), mouse anti-Wg (1:50, DSHB), guinea pig anti-PromL (1:150, this study), mouse anti Dll44 (1:100), rabbit anti-aPKC (1:500, Santa Cruz), rat anti-DE-cadherin (1:50, DSHB), mouse anti-Dlg (1:100, DSHB);mouse anti-Arm (1:100,DSHB),mouse anti-GFP (1:1000, Roche), rabbit anti-Cad99c25 (1:1000, D. Godt, University of Toronto), rabbit anti-Caspase3 (1:500,CellSignaling).
Article Title: Body-fat sensor triggers ribosome maturation in the steroidogenic gland to initiate sexual maturation in Drosophila.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-Dlg DSHB Cat#4F3; RRID: AB_528203 mouse anti-Fibrillarin ThermoFisher Scientific Cat#38F3; RRID: AB_2532241 mouse anti-Lam Dm0 DSHB Cat#ADL84.12; RRID:AB_528338 mouse anti-Wg DSHB Cat#4D4; RRID: AB_528512 rabbit anti-Apolpp Joaquin Culi, Kutty et al., 1996 N/A rabbit anti-GFP Abcam Cat#Ab290 rabbit anti-IgG HRP-conjugated Sigma-Aldrich Cat# A9169 rabbit anti-ILP2 Hugo Stocker, Bader et al., 2013 N/A rabbit anti-Sema1a Alex Kolodkin, Yu et al., 1998 N/A rat anti-DE-Cad DSHB Cat#DCAD2; RRID: AB_528120 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31570; RRID: AB_2536180 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31572; RRID: AB_162543 donkey Anti-Rat IgG (H+L) Alexa Fluor 647 AffiniPure Jackson ImmunoResearch Cat#712-605-153; RRID: AB_2340694 Chemicals, peptides, and recombinant proteins Vectashield mounting medium with DAPI Vector Labs Cat#H-1200 DAPI-Fluoromount-G Electron Microscopy Sciences Cat#17984-24 GIBCO Schneider’s Drosophila Sterile Medium Thermo Fisher Cat#21720024 Bromophenol blue Sigma-Aldrich Cat#B6131 a-ecdysone Sigma-Aldrich Cat#9004 20-hydroxyecdysone Sigma-Aldrich Cat#H5142 SuperScript III First-Strand Synthesis System for RT-PCR Invitrogen Cat#18080-051 Power SYBR Green PCR Master Mix Applied Biosystems Cat#4367659 Paraformaldehyde 16% solution Electron Microscopy Sciences Cat#15710 Nile Red Sigma-Aldrich Cat#72485 1X cOmplete Mini EDTAfree protease inhibitor cocktail Sigma-Aldrich Cat#11836170001 Phalloidin 633 ThermoFisher Cat#A22284 Critical commercial assays 20-Hydroxyecdysone enzyme immunoassay kit Bertin Pharma Cat#A05120 Amplex Red Cholesterol Assay Kit ThermoFisher Scientific Cat#A12216 BCA Protein Assay Kit Pierce Cat#23227 Glucose (HK) Assay Kit Sigma-Aldrich Cat#GAHK20-1KT RNeasy-Mini Kit QIAGEN Cat#74106 RNase Free DNase Set QIAGEN Cat#79254 Experimental models: Organisms/strains D. melanogaster: mir-8NP5247-Gal4 DGRC 104917 D. melanogaster: NP2222-Gal4 DRGC 112830 D. melanogaster: phm22-Gal4 Kim Rewitz, Ono et al., 2006 N/A (Continued on next page) Cell Reports 37, 109830, October 12, 2021 e1
Article Title:
Article Snippet: Mouse anti-Antennapedia Developmental State Hybridoma Bank, USA Cat# DSHB 8C11, RRID: AB_528083 Mouse anti-Wg Developmental State Hybridoma Bank, USA Cat# DSHB 4D4, RRID:AB_528512 Rat anti-Ci Developmental State Hybridoma Bank, USA Cat# DSHB 2A1, RRID:AB_2109711 Rabbit anti-pMad Abcam Cat# ab52903, RRID:AB_882596 Mouse anti-phosphoCaMKII ThermoFisher Scientific Cat# MA1-047, RRID: AB_325402 Mouse anti-P1 Gift from Dr. Istvan Ando, Hungarian Academy of Sciences, Hungary N/A
Article Title: Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling
Article Snippet: Primary antibodies were used for staining were: Mouse anti-V5 (Invitrogen R960-25; preabsorbed at 1:10 dilution, then used at 1:50), Rat anti-E-cadherin (DSHB DCAD2-c 1:200), Chicken anti-ß-gal (Abcam ab9361; 1:100), Rat anti-Fat (1:1500) , Mouse anti-Wg (DSHB 9A4; 1:300), Rabbit anti-Dcr2 (Abcam ab4732, 1:1600), Rabbit anti-FLAG (Sigma F3165; 1:200).
Article Title: InR and Pi3K maintain intestinal homeostasis through STAT/EGFR and Notch signaling in enteroblasts.
Article Snippet: Funding information Fundamental Research Funds for the Central Universities, Grant/Award Number: 2572022DQ07; National Natural Science Foundation of China, Grant/Award Number: 32170484 Abstract To maintain the integrity of the adult gut, the proliferation and differentiation of stem cells must be strictly controlled.. Several signaling pathways control the proliferation and differentiation of Drosophila intestinal epithelial cells.. Although the modulatory effects of insulin pathway components on cell proliferation have been characterized, their specific role in which cell type and how these components interact with other regulatory signaling pathways remain largely unclear.
Bicinchoninic Acid Protein Assay:Article Title: Nuclear receptor E75/NR1D2 drives tumor malignant transformation by integrating Hippo and Notch pathways
Article Snippet: Primary antibodies used include mouse Anti-beta Galactosidase (1:100, DSHB), mouse anti-Br (1:100, DSHB), rat anti- Elav (1:100, DSHB), mouse anti-Mmp-1 (1:100, DSHB), mouse anti-wg (1:100, DSHB), Cyclin E (1:100, Santa Cruz), rabbit anti-Dcp1 (CST, 1:100), mouse anti-dMyc (1:50, DSHB), mouse anti-Cut (1:100, DSHB), mouse anti-NICD (1:100, DSHB).
Article Title: Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila.
Article Snippet: This article is protected by copyright.. All rights reserved.. A cc ep te d A rt ic le Ashim Mukherjee ORCID iD: 0000-0002-3523-7089 Deltex cooperates with TRAF6 to promote apoptosis and cell migration through Eiger-independent JNK activation in Drosophila Vartika Sharma, Mousumi Mutsuddi, Ashim Mukherjee* Department of Molecular and Human Genetics, Institute of Science, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India *Corresponding Author Email: amukherjee@bhu.ac.in
Article Title: Microvilli-derived extracellular vesicles carry Hedgehog morphogenic signals for Drosophila wing imaginal disc development.
Article Snippet: Antibodies were used as follows: mouse anti-En (1:1000, Development Studies Hybridoma Bank University of Iowa – DSHB), rabbit anti-En (1:1000, Santa Cruz), mouse anti-Ptc42 (1/400), chicken anti-bgal (1:1000, Gen Tex), affinity-purified rabbit ‘‘Calvados’’ polyclonal anti-Hh43 (1:200), mouse anti-Cut (1:100, DSHB), mouse anti-Wg (1:50, DSHB), guinea pig anti-PromL (1:150, this study), mouse anti Dll44 (1:100), rabbit anti-aPKC (1:500, Santa Cruz), rat anti-DE-cadherin (1:50, DSHB), mouse anti-Dlg (1:100, DSHB);mouse anti-Arm (1:100,DSHB),mouse anti-GFP (1:1000, Roche), rabbit anti-Cad99c25 (1:1000, D. Godt, University of Toronto), rabbit anti-Caspase3 (1:500,CellSignaling).
Article Title: Body-fat sensor triggers ribosome maturation in the steroidogenic gland to initiate sexual maturation in Drosophila.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-Dlg DSHB Cat#4F3; RRID: AB_528203 mouse anti-Fibrillarin ThermoFisher Scientific Cat#38F3; RRID: AB_2532241 mouse anti-Lam Dm0 DSHB Cat#ADL84.12; RRID:AB_528338 mouse anti-Wg DSHB Cat#4D4; RRID: AB_528512 rabbit anti-Apolpp Joaquin Culi, Kutty et al., 1996 N/A rabbit anti-GFP Abcam Cat#Ab290 rabbit anti-IgG HRP-conjugated Sigma-Aldrich Cat# A9169 rabbit anti-ILP2 Hugo Stocker, Bader et al., 2013 N/A rabbit anti-Sema1a Alex Kolodkin, Yu et al., 1998 N/A rat anti-DE-Cad DSHB Cat#DCAD2; RRID: AB_528120 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31570; RRID: AB_2536180 donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#A-31572; RRID: AB_162543 donkey Anti-Rat IgG (H+L) Alexa Fluor 647 AffiniPure Jackson ImmunoResearch Cat#712-605-153; RRID: AB_2340694 Chemicals, peptides, and recombinant proteins Vectashield mounting medium with DAPI Vector Labs Cat#H-1200 DAPI-Fluoromount-G Electron Microscopy Sciences Cat#17984-24 GIBCO Schneider’s Drosophila Sterile Medium Thermo Fisher Cat#21720024 Bromophenol blue Sigma-Aldrich Cat#B6131 a-ecdysone Sigma-Aldrich Cat#9004 20-hydroxyecdysone Sigma-Aldrich Cat#H5142 SuperScript III First-Strand Synthesis System for RT-PCR Invitrogen Cat#18080-051 Power SYBR Green PCR Master Mix Applied Biosystems Cat#4367659 Paraformaldehyde 16% solution Electron Microscopy Sciences Cat#15710 Nile Red Sigma-Aldrich Cat#72485 1X cOmplete Mini EDTAfree protease inhibitor cocktail Sigma-Aldrich Cat#11836170001 Phalloidin 633 ThermoFisher Cat#A22284 Critical commercial assays 20-Hydroxyecdysone enzyme immunoassay kit Bertin Pharma Cat#A05120 Amplex Red Cholesterol Assay Kit ThermoFisher Scientific Cat#A12216 BCA Protein Assay Kit Pierce Cat#23227 Glucose (HK) Assay Kit Sigma-Aldrich Cat#GAHK20-1KT RNeasy-Mini Kit QIAGEN Cat#74106 RNase Free DNase Set QIAGEN Cat#79254 Experimental models: Organisms/strains D. melanogaster: mir-8NP5247-Gal4 DGRC 104917 D. melanogaster: NP2222-Gal4 DRGC 112830 D. melanogaster: phm22-Gal4 Kim Rewitz, Ono et al., 2006 N/A (Continued on next page) Cell Reports 37, 109830, October 12, 2021 e1
Article Title:
Article Snippet: Mouse anti-Antennapedia Developmental State Hybridoma Bank, USA Cat# DSHB 8C11, RRID: AB_528083 Mouse anti-Wg Developmental State Hybridoma Bank, USA Cat# DSHB 4D4, RRID:AB_528512 Rat anti-Ci Developmental State Hybridoma Bank, USA Cat# DSHB 2A1, RRID:AB_2109711 Rabbit anti-pMad Abcam Cat# ab52903, RRID:AB_882596 Mouse anti-phosphoCaMKII ThermoFisher Scientific Cat# MA1-047, RRID: AB_325402 Mouse anti-P1 Gift from Dr. Istvan Ando, Hungarian Academy of Sciences, Hungary N/A
Article Title: Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling
Article Snippet: Primary antibodies were used for staining were: Mouse anti-V5 (Invitrogen R960-25; preabsorbed at 1:10 dilution, then used at 1:50), Rat anti-E-cadherin (DSHB DCAD2-c 1:200), Chicken anti-ß-gal (Abcam ab9361; 1:100), Rat anti-Fat (1:1500) , Mouse anti-Wg (DSHB 9A4; 1:300), Rabbit anti-Dcr2 (Abcam ab4732, 1:1600), Rabbit anti-FLAG (Sigma F3165; 1:200).
Article Title: InR and Pi3K maintain intestinal homeostasis through STAT/EGFR and Notch signaling in enteroblasts.
Article Snippet: Funding information Fundamental Research Funds for the Central Universities, Grant/Award Number: 2572022DQ07; National Natural Science Foundation of China, Grant/Award Number: 32170484 Abstract To maintain the integrity of the adult gut, the proliferation and differentiation of stem cells must be strictly controlled.. Several signaling pathways control the proliferation and differentiation of Drosophila intestinal epithelial cells.. Although the modulatory effects of insulin pathway components on cell proliferation have been characterized, their specific role in which cell type and how these components interact with other regulatory signaling pathways remain largely unclear.
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